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. 2017 Aug 8;73(1):49–61. doi: 10.1136/thoraxjnl-2017-210298

Figure 3.

Figure 3

LasB reduces trappin-2 and IL-6 recovery, but not that of IL-8, in the supernatants of NCI-H292, Calu-3 and CFBE cells (WT-CFTR and ΔF508). (A–C): NCI-H292 cells were preincubated with IL-1β for 1 hour prior to the addition of diluted SEC (5%) or purified LasB (pLasB) for 4 hours, supplemented or not with the metalloprotease inhibitor phosphoramidon (PA, 8.5 µM). DMSO was also added as a control, since it was used as a diluent for PA. Trappin-2, IL-6 and IL-8 protein levels were measured by ELISA in cell supernatants (n=4, *p<0.05, **p<0.01, ***p<0.001, ns: not significant; ANOVA). (D–F): Calu-3 cells were incubated with LB medium or with diluted SECs (5%) for 4 hours. Trappin-2, IL-6 and IL-8 protein levels were measured as above (n=3, ANOVA). (G–I): CFBE-WT-CFTR and CFBE-F508del-CFTR cells were either untreated (CTL), treated with LB medium, or with diluted SEC (1%) for 24 hours and ELISAs performed as above (n=3–4, two-way ANOVA). Results are shown as mean±SEM. ANOVA, analysis of variance; CFBE, cystic fibrosis bronchial epithelial; CFTR, cystic fibrosis transmembrane conductance regulator; DMSO, dimethyl sulfoxyde; IL; interleukin; LB, Luria Broth; SEC, secretomes.