Figure 3. An example of marker development by RNA-Seq. (A) eibi1 genetic map. Bmag828 and Bmag603 are SSR markers. The others are EST markers. A total of 155 F₂ individuals derived from the cross of Morex eibi1 were used for the genetic mapping. The numbers at the left side indicate genetic distances in centimorgan (cM). (B) Corresponding marker polymorphism between parental line Morex and eibi1. The polymorphisms were identified by PCR sequencing against parental genomic DNA. (C) Corresponding eibi1 Unigenes (derived from RNA-Seq) for the EST markers. Inside the parenthesis is a nucleotide followed by its position in the corresponding Unigene sequence. (D) Corresponding Morex contigs for the EST markers were retrieved from the database: assembly3_WGSMorex_renamed_blastable_POPSEQ.fasta (http://webblast.ipk-gatersleben.de/barley). Inside the parenthesis is a nucleotide followed by its position in the corresponding contig sequence.