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. 2017 Dec 8;50(12):2958–2967. doi: 10.1021/acs.accounts.7b00445

Figure 3.

Figure 3

Synthetic targets used to probe antibodies present in infected cattle and in mice immunized with glycoconjugate vaccine 12d. M tetrasaccharide 7a, disaccharide 8a, and the two-component trisaccharides 9a and 10a found in the M repeating sequence. M and A hexasaccharides 11a and 12a represent larger epitopes. Trisaccharide 13a represents a small A-type epitope. Oligosaccharide amides 7b–13b were conjugated to BSA to provide glycoconjugates for use in ELISA. Hexasaccharide 12a was derivatized, conjugated to tetanus toxoid, and then used to immunize mice.