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. 2017 Dec 8;50(12):2958–2967. doi: 10.1021/acs.accounts.7b00445

Figure 4.

Figure 4

Heptasaccharide 27 was synthesized as the amine and conjugated to tetanus toxoid using disuccinimidyl glutarate (DSG) to obtain 28 and to BSA using diethyl squarate to obtain 29. Conjugate 28 was used to immunize mice and conjugate 29 to monitor antibody responses by ELISA.