Figure 6.

Intermittent long‐term (56 days) everolimus treatment rescues mTORC1 sensitivity to therapy in vivo. GFP‐LC3 mice were administered 3 mg·kg⁻¹ everolimus (EVR) by oral gavage every other day for a period of 56 days. At the end of the experiment, mice were then given an I.P. injection containing 100 mg·kg⁻¹ chloroquine and were killed 3 h later. Western blotting was performed to measure the phosphorylation status of Akt1 (Ser⁴⁷³), mTOR (Ser²⁴⁴⁸) and S6rp (Ser^235/236) as well as the expression of Cdk2. ACTB was used as a loading control. Representative blots are shown (n = 6 for both groups, *P < 0.05, significantly different from vehicle).