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. 2016 Nov 1;173(23):3342–3358. doi: 10.1111/bph.13623

Figure 4.

Figure 4

Effects of AZM475271 and PP2 on SFK and FAK phosphorylation. Detection of Y416‐phosphorylated SFKs, total c‐Src, Y397‐ and Y925‐phosphorylated FAK, total FAK and β‐actin was performed by western blot using site‐ and phospho‐specific antibodies. Content of phosphorylated and total proteins was semiquantitatively compared between tissues or cells incubated with DMSO (control) or inhibitors. Application of AZM475271 or PP2 (10 μM each) reduced SFK phosphorylation at tyrosine 416 in prostate tissues and WPMY‐1 cells (A). In contrast, AZM475271 or PP2 (10 μM each) were without effect on FAK phosphorylation at both positions (Y397 and Y925) in prostate tissues (B). Shown are representative western blots and quantification of all experiments (series with n = 6 independent experiments and different prostates, respectively, or with n = 6 independent experiments with WPMY‐1 cells) (means ± SEM).