Figure 1.

Activation of A_2A receptors rapidly increases cellular β‐catenin levels. (A) NHDF cells were incubated with CGS21680 (1 μM) for periods of up to 4 h and collected at the indicated time‐points. (B) NHDF cells were incubated with increasing concentrations of CGS21680 (0.1–10 μM) for 15 min, with or without SCH58261 (1 μM) pre‐incubation. Cellular β‐catenin levels were measured. Representative images of western blots for β‐catenin and actin are shown. Data represent mean ± SEM of six independent experiments as determined by densitometry relative to actin. *P < 0.05 versus non‐stimulated control; #P < 0.05, SCH58261 + CGS26180 versus CGS26180. CGS indicates CGS26180.