Figure 3.

SAHA effects on HuR expression in ATC cells. (A) Relative expression levels of HuR mRNA after SAHA 3 µM or DMSO treatment for 48 h. RNA extraction and qPCR are described in Materials and method section. For each cell line, the results were normalized against β-actin levels and expressed in arbitrary unit, calculated as described in Materials and Methods section. (B) Western blot analysis of HuR expression in SW1736 and 8505C treated with SAHA 3 µM or DMSO for 72 h. (C) Densitometric analysis of HuR protein levels in ATC cell lines treated with SAHA or DMSO. For each cell line, the results were normalized against β-actin levels and expressed in arbitrary unit. Results are shown as mean ± standard deviation. *P<0.05 by Student's t-test.