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Effect of PGE2 on the level of PD-1 expression. PGE2 was added into the culture of sorted CD8+ T cells and a dose- and time-dependent effect of PGE2 on the PD-1 expression level was confirmed by (A) quantitative polymerase chain reaction and (B) western blot analysis. Data is presented as the mean ± standard deviation. *P<0.05. Con, control group with no PGE2 added; PGE2, prostaglandin E2; PD-1, programmed cell death 1; CD, cluster of differentiation.
