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. 2017 Oct 26;15(1):552–558. doi: 10.3892/ol.2017.7279

Table II.

Primer sequences used in quantitative polymerase chain reaction.

Primer Primer sequences (5′-3′)
PD-1
  Forward AAGCTTATGTGGGTCCGGC
  Reverse GGATCCTCAAAGAGGCC
EP1
  Forward TCGCTTCGGCCTCCACCTTCTTTG
  Reverse CGTTGGGCCTCTGGTTGTGCTTAG
EP2
  Forward CCACGATGCTCCTGCTGCTT
  Reverse TCCACAAAGGTCAGTCTGTTT
EP3
  Forward CGGGGCTACGGAGGGGATGC
  Reverse ATGGCGCTGGCGATGAACAACGAG
EP4
  Forward GGTCATCTTACTCATCGCCACCTCTC
  Reverse TCCCACTAACCTCATCCACCAACAG
GAPDH
  Forward GGTGGTCTCCTCTGACTTCAACA
  Reverse GTGGTCGTTGAGGGCAATG

PD-1, programmed cell death 1; EP, prostaglandin E receptor.