Expression levels of 5mC, 5hmC, TET1 and TET2 were analyzed using IHC in gastric cancer tissue arrays. A representative case is presented in this figure. (A) The IHC assay analyzed the 5mC and 5hmC expression levels in a patient with gastric cancer (magnification, ×100), and (B) the relative 5mC and 5hmC expression levels were scored between gastric cancer and adjacent normal tissues from 58 patients. (C) The IHC assay revealed the expression levels of TET1 and TET2 in a patient with gastric cancer (magnification, ×100). (D) The relative expression levels of TET1 and TET2 were scored between the gastric cancer tissues of 58 patients and their corresponding adjacent normal tissues. IHC, immunohistochemistry; TET, ten-eleven translocation; 5mC, 5-methylcytosine; 5hmC, 5-hydroxymethylcytosine.