Figure 4.

TET1 is involved in active DNA demethylation pathway in AGS cells. (A) Schematic diagrams of TET1 expression constructs for TET1-full length and TET1-delNLS. AGS cells were co-transfected with methylated GFP or unmethylated GFP, and TET1-FL or TET1-delNLS. Following transfection for 24 h, the GFP expression was detected by (B) fluorescence microscopy (magnification, ×100) and (C) western blotting. (D) The intensity of GFP was quantified using ImageJ software and was represented graphically. **P<0.01. Actin was used as an internal control. TET, ten-eleven translocation; GFP, green fluorescent protein; NES, nuclear export signal; NLS, nuclear localization signals; TET1-FL, full length of TET1 genes; TET1-delNLS, NLS domain of TET1 was deleted.