Table 2.
Method | Advantages | Disadvantages |
---|---|---|
Cloning in phage vector | Uses standard cloning techniques Does not require a selection target or a phage display library Phage clones are well defined molecularly Phage clones remain viable for extended periods when properly stored |
Nucleotide sequence encoding fusion peptide must be known Size and composition of fusion peptide depend on phage vector Phage clones can be genetically unstable and/or have low propagation rate, depending on phage vector, size and composition of fusion peptide |
Selection from phage display library | Selection procedure is relatively simple Phage clones are genetically stable and propagate well Phage clones are well defined molecularly Phage clones remain viable for extended periods when properly stored |
Selection target has to be available and well characterized Appropriate phage display library has to be available |
Chemical conjugation to phage coat proteins | Uses standard conjugation chemistries Flexible as to size and composition of fusion peptides Relatively chemically stable if stored properly |
Number of fusion peptide copies per phage particle varies between batches Conjugation sites of fusion peptides are not specific |