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. 2017 Oct 30;15(1):338–346. doi: 10.3892/ol.2017.7283

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Copyright: © Lu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — Two-dimensional gel electrophoresis of NMPs in MG-63 cells treated with or without curcumin. (A) Two-dimensional 11 cm, pH 4–7 immobilized pH gradient strips were used for isoelectric focusing. Protein spot 36 was identified as 14-3-3ε. (B) Partial enlargement image of spot 36. (C) Quantification of spot 36 in Con and CUR; maximal value was 7,681 PPM. Using PDQuest software, spot quantity=spot height × π × σ_x × σ_y (INT*area). PPM, parts per million; Con, control; CUR, curcumin-treated; INT, intensity. Normalization method, total quantity in valid spots.