Table 2.

Two-way ANCOVA results of cellular immune parameter from brooding vs. non-brooding females: Significant p values (p < 0.05) are marked in bold letters. Results from Tukey HSD posthoc tests can be found in Additional file 2: Table S2

		Blood				Spleen				Head Kidney
	Df	SS	MS	F value	Pr(>F)	SS	MS	F value	Pr(>F)	SS	MS	F value	Pr(>F)
Lymphocyte/Monocyte
Treatment	1	0.017	0.017	0.89	0.378	0.70	0.70	2.11	0.190	4.30	4.30	5.62	0.050
Challenge	1	0.008	0.008	0.43	0.531	0.07	0.07	0.20	0.667	0.07	0.07	0.09	0.775
Condition factor	1	0.018	0.018	0.94	0.364	0.01	0.01	0.03	0.861	0.10	0.10	0.13	0.733
Treatment*Challenge	1	0.006	0.006	0.34	0.580	0.07	0.07	0.21	0.660	0.02	0.02	0.02	0.887
Residuals	7	0.132	0.019			2.32	0.33			5.37	0.77
Active/Inactive Cells
Treatment	1	0.000	0.000	3.32	0.1113	0.51	0.51	0.54	0.488	0.00	0.00	7.29	0.031
Challenge	1	0.000	0.000	0.00	0.9643	0.23	0.23	0.24	0.638	0.00	0.00	0.08	0.783
Condition factor	1	0.000	0.000	0.13	0.7311	1.10	1.10	1.17	0.316	0.00	0.00	5.84	0.046
Treatment*Challenge	1	0.000	0.000	0.01	0.9433	0.05	0.04	0.05	0.834	0.00	0.00	0.40	0.546
Residuals	7	0.000	0.000			6.63	0.95			0.00	0.00