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. 2017 Dec 20;17:264. doi: 10.1186/s12862-017-1109-6

Table 6.

nested MANOVA results of gene expression analysis of mouthbred juveniles: nested MANOVA to asses effects of maternal challenge, juvenile challenge and their interaction on the relative expression of candidate genes (ΔCt values) in mouthbred juveniles. Female challenge was nested in family. Significant results are marked in bold letters. Results of the univariate posthoc analysis (ANCOVA & Tukey HSD) can be found in Additional file 7: Table S7 and Additional file 8: Table S8

nested MANOVA All genes All immune genes Adaptive genes
Factors DF Pillai F n Df d Df Pr(>F) Pillai F n Df d Df Pr(>F) Pillai F n Df d Df Pr(>F)
Juvenile Challenge (jC) 2 1.98 5.35 90 4 0.055 1.16 1.11 52 42 0.362 0.37 0.97 18 76 0.497
Maternal Challenge (mC) 1 1.00 2972.31 45 1 0.015 0.86 4.67 26 20 < 0.001 0.28 1.60 9 37 0.152
jC * mC 2 1.92 1.03 90 4 0.571 1.20 1.21 52 42 0.263 0.31 0.77 18 76 0.726
mC in family 4 3.95 6.87 180 16 0.000 2.85 2.20 104 92 < 0.001 1.24 2.01 36 160 0.002
Residuals 45
All innate IS Inflammation Oxidative Stress
Factors DF Pillai F n Df d Df Pr(>F) Pillai F n Df d Df Pr(>F) Pillai F n Df d Df Pr(>F)
Juvenile Challenge (jC) 2 0.75 1.27 30 64 0.211 0.34 1.72 10 84 0.089 0.19 1.50 6 88 0.188
Maternal Challenge (mC) 1 0.80 8.45 15 31 < 0.001 0.28 3.25 5 41 0.015 0.05 0.68 3 43 0.568
jC * mC 2 0.58 0.88 30 64 0.643 0.25 1.18 10 84 0.318 0.13 1.05 6 88 0.398
mC in family 4 1.90 2.06 60 136 < 0.001 0.50 1.26 20 176 0.212 0.39 1.66 12 135 0.082
Residuals 45
Various innate IS Antimicrobial Peptides Complement Components
Factors DF Pillai F n Df d Df Pr(>F) Pillai F n Df d Df Pr(>F) Pillai F n Df d Df Pr(>F)
Juvenile Challenge (jC) 2 0.29 1.84 8 86 0.080 0.22 2.84 4 90 0.029 0.77012 1.105 34 60 0.361
Maternal Challenge (mC) 1 0.15 1.88 4 42 0.132 0.06 1.30 2 44 0.283 0.80387 6.992 17 29 < 0.001
jC * mC 2 0.14 0.84 8 86 0.571 0.05 0.59 4 90 0.667 0.64951 0.8487 34 60 0.693
mC in family 4 0.61 2.04 16 180 0.013 0.25 1.58 8 90 0.143 2.19249 2.2833 68 128 < 0.001
Residuals 45
Epigenetic genes Developmental genes Stress related genes
Factors DF Pillai F n Df d Df Pr(>F) Pillai F n Df d Df Pr(>F) Pillai F n Df d Df Pr(>F)
Juvenile Challenge (jC) 2 0.25 1.19 10 84 0.306 0.23 1.40 8 86 0.208 0.28 1.76 8 86 0.097
Maternal Challenge (mC) 1 0.31 3.73 5 41 0.007 0.16 1.93 4 42 0.122 0.01 0.13 4 42 0.972
jC * mC 2 0.37 1.90 10 84 0.056 0.21 1.27 8 86 0.268 0.19 1.10 8 86 0.374
mC in family 4 0.70 1.88 20 176 0.017 0.54 1.74 16 180 0.042 0.29 0.89 16 180 0.586
Residuals 45
Metabolism genes Sex related genes
Factors DF Pillai F n Df d Df Pr(>F) Pillai F n Df d Df Pr(>F)
Juvenile Challenge (jC) 2 0.13 1.02 6 88 0.418 0.29179 2.5053 6 88 0.027622
Maternal Challenge (mC) 1 0.17 2.91 3 43 0.045 0.2595 5.0231 3 43 0.004501
jC * mC 2 0.06 0.49 6 88 0.816 0.12935 1.0142 6 88 0.421418
mC in family 4 0.32 1.35 12 135 0.196 0.20872 0.8412 12 135 0.608014
Residuals 45