Table 6.
nested MANOVA results of gene expression analysis of mouthbred juveniles: nested MANOVA to asses effects of maternal challenge, juvenile challenge and their interaction on the relative expression of candidate genes (ΔCt values) in mouthbred juveniles. Female challenge was nested in family. Significant results are marked in bold letters. Results of the univariate posthoc analysis (ANCOVA & Tukey HSD) can be found in Additional file 7: Table S7 and Additional file 8: Table S8
nested MANOVA | All genes | All immune genes | Adaptive genes | |||||||||||||
Factors | DF | Pillai | F | n Df | d Df | Pr(>F) | Pillai | F | n Df | d Df | Pr(>F) | Pillai | F | n Df | d Df | Pr(>F) |
Juvenile Challenge (jC) | 2 | 1.98 | 5.35 | 90 | 4 | 0.055 | 1.16 | 1.11 | 52 | 42 | 0.362 | 0.37 | 0.97 | 18 | 76 | 0.497 |
Maternal Challenge (mC) | 1 | 1.00 | 2972.31 | 45 | 1 | 0.015 | 0.86 | 4.67 | 26 | 20 | < 0.001 | 0.28 | 1.60 | 9 | 37 | 0.152 |
jC * mC | 2 | 1.92 | 1.03 | 90 | 4 | 0.571 | 1.20 | 1.21 | 52 | 42 | 0.263 | 0.31 | 0.77 | 18 | 76 | 0.726 |
mC in family | 4 | 3.95 | 6.87 | 180 | 16 | 0.000 | 2.85 | 2.20 | 104 | 92 | < 0.001 | 1.24 | 2.01 | 36 | 160 | 0.002 |
Residuals | 45 | |||||||||||||||
All innate IS | Inflammation | Oxidative Stress | ||||||||||||||
Factors | DF | Pillai | F | n Df | d Df | Pr(>F) | Pillai | F | n Df | d Df | Pr(>F) | Pillai | F | n Df | d Df | Pr(>F) |
Juvenile Challenge (jC) | 2 | 0.75 | 1.27 | 30 | 64 | 0.211 | 0.34 | 1.72 | 10 | 84 | 0.089 | 0.19 | 1.50 | 6 | 88 | 0.188 |
Maternal Challenge (mC) | 1 | 0.80 | 8.45 | 15 | 31 | < 0.001 | 0.28 | 3.25 | 5 | 41 | 0.015 | 0.05 | 0.68 | 3 | 43 | 0.568 |
jC * mC | 2 | 0.58 | 0.88 | 30 | 64 | 0.643 | 0.25 | 1.18 | 10 | 84 | 0.318 | 0.13 | 1.05 | 6 | 88 | 0.398 |
mC in family | 4 | 1.90 | 2.06 | 60 | 136 | < 0.001 | 0.50 | 1.26 | 20 | 176 | 0.212 | 0.39 | 1.66 | 12 | 135 | 0.082 |
Residuals | 45 | |||||||||||||||
Various innate IS | Antimicrobial Peptides | Complement Components | ||||||||||||||
Factors | DF | Pillai | F | n Df | d Df | Pr(>F) | Pillai | F | n Df | d Df | Pr(>F) | Pillai | F | n Df | d Df | Pr(>F) |
Juvenile Challenge (jC) | 2 | 0.29 | 1.84 | 8 | 86 | 0.080 | 0.22 | 2.84 | 4 | 90 | 0.029 | 0.77012 | 1.105 | 34 | 60 | 0.361 |
Maternal Challenge (mC) | 1 | 0.15 | 1.88 | 4 | 42 | 0.132 | 0.06 | 1.30 | 2 | 44 | 0.283 | 0.80387 | 6.992 | 17 | 29 | < 0.001 |
jC * mC | 2 | 0.14 | 0.84 | 8 | 86 | 0.571 | 0.05 | 0.59 | 4 | 90 | 0.667 | 0.64951 | 0.8487 | 34 | 60 | 0.693 |
mC in family | 4 | 0.61 | 2.04 | 16 | 180 | 0.013 | 0.25 | 1.58 | 8 | 90 | 0.143 | 2.19249 | 2.2833 | 68 | 128 | < 0.001 |
Residuals | 45 | |||||||||||||||
Epigenetic genes | Developmental genes | Stress related genes | ||||||||||||||
Factors | DF | Pillai | F | n Df | d Df | Pr(>F) | Pillai | F | n Df | d Df | Pr(>F) | Pillai | F | n Df | d Df | Pr(>F) |
Juvenile Challenge (jC) | 2 | 0.25 | 1.19 | 10 | 84 | 0.306 | 0.23 | 1.40 | 8 | 86 | 0.208 | 0.28 | 1.76 | 8 | 86 | 0.097 |
Maternal Challenge (mC) | 1 | 0.31 | 3.73 | 5 | 41 | 0.007 | 0.16 | 1.93 | 4 | 42 | 0.122 | 0.01 | 0.13 | 4 | 42 | 0.972 |
jC * mC | 2 | 0.37 | 1.90 | 10 | 84 | 0.056 | 0.21 | 1.27 | 8 | 86 | 0.268 | 0.19 | 1.10 | 8 | 86 | 0.374 |
mC in family | 4 | 0.70 | 1.88 | 20 | 176 | 0.017 | 0.54 | 1.74 | 16 | 180 | 0.042 | 0.29 | 0.89 | 16 | 180 | 0.586 |
Residuals | 45 | |||||||||||||||
Metabolism genes | Sex related genes | |||||||||||||||
Factors | DF | Pillai | F | n Df | d Df | Pr(>F) | Pillai | F | n Df | d Df | Pr(>F) | |||||
Juvenile Challenge (jC) | 2 | 0.13 | 1.02 | 6 | 88 | 0.418 | 0.29179 | 2.5053 | 6 | 88 | 0.027622 | |||||
Maternal Challenge (mC) | 1 | 0.17 | 2.91 | 3 | 43 | 0.045 | 0.2595 | 5.0231 | 3 | 43 | 0.004501 | |||||
jC * mC | 2 | 0.06 | 0.49 | 6 | 88 | 0.816 | 0.12935 | 1.0142 | 6 | 88 | 0.421418 | |||||
mC in family | 4 | 0.32 | 1.35 | 12 | 135 | 0.196 | 0.20872 | 0.8412 | 12 | 135 | 0.608014 | |||||
Residuals | 45 |