Fig. 5.

The dendritic cells in the spleen after LPS-induced sepsis in mice. a The gating strategies for analyzing the dendritic cells and dendritic cell subsets. b The percentage of CD11c⁺ cells among all live splenocytes at 6 h, 24 h, 3 days, and 7 days in naive, saline-treated, and LPS-treated mice. c–f The percentage of different dendritic cell subsets among the total cDCs (CD11c⁺) in the mouse spleen. g The percentage of different cDC subpopulations among the total live splenocytes in the normal WT mice. h–l The percentage of total cDCs among the total live splenocytes (h) and different subpopulations of cDCs among the total cDCs (i–l) in the WT mouse spleen after LPS treatment. At 6 h: naive = 6 mice, saline = 7 mice, LPS = 12 mice; at 24 h: naive = 6 mice, saline = 5 mice, LPS = 7 mice; at 3 days: naive = 5 mice, saline = 6 mice, LPS = 7 mice; at 7 days: naive = 5 mice, saline = 6 mice, LPS = 7 mice. Data are presented as means ± SD; asterisk, LPS vs. naive; hashtag, LPS vs. saline; *p < 0.05, **p < 0.01, ***p < 0.001, ^# p < 0.05, ^## p < 0.01, ^### p < 0.001, by two-way ANOVA with LSD for pairwise comparisons