Fig. 6.

Nicotine fails to stimulate NSCLC cell proliferation alone or interfere with paclitaxel-induced growth inhibition of NSCLC cells. The ‘start’ time point represents the initial number of A549 cells (left) or H460 cells (right) after seeding. A 24-hour nicotine pretreatment period occurred from start to day 0 for the nicotine and Pac + Nic conditions, then all subsequent treatments lasted 24 hours; no drugs were present after day 1. The number of cells was determined via trypan blue exclusion. ****P < 0.0001 versus control. Data are expressed as the mean ± S.E.M. of three independent experiments.