TABLE 1.
Class and suppressor | Genotype | Strain |
---|---|---|
Class I (FlgM loss-of-function mutations) | ||
sod1 | flgMQ31^FS | DS6697 |
sod2 | flgMQ64* | DS6698 |
sod3 | flgMQ31^FS | DS6699 |
sod5 | flgMN17^FS | DS7528 |
sod6 | flgMV72* | DS7529 |
sod7 | flgMQ24* | DS7530 |
sod8 | flgMQ64* | DS8791 |
sod39 | flgMQ9* | DK39 |
sod40 | flgMQ64* | DK40 |
sod41 | flgMQ64* | DK41 |
sod42 | flgMQ64* | DK42 |
sod43 | flgMQ64* | DK43 |
sod44 | flgMT26^FS | DK44 |
sod46 | flgMN80^FS | DK46 |
sod47 | flgMQ64* | DK47 |
sod51 | flgMQ64* | DK615 |
sod52 | flgMQ64* | DK616 |
Class II (PfliD promoter-down mutation): sod4 | PfliD TGTAAT→CGTAAT | DS7527 |
Class III (FliS translation-down mutation): sod48 | fliSRBS GGAGG→AGAGG | DK48 |
Unidentified | ||
sod38 | Unknown | DK38 |
sod50 | Unknown | DK614 |
Each sod mutation was independently isolated from a separate swarm plate, each inoculated with a separate colony of the DS6657 ΔswrD parent. Thus, although some of the mutations were identical, they are not siblings and represent independent genetic events. ^FS, frameshift after the indicated codon; *, stop codon. Bold letters indicate the nucleotide position changed by the mutation. Underlined letters indicate the −35 element of the PfliD σD-dependent promoter. RBS, ribosome binding site or Shine-Dalgarno sequence.