FIG 2.

Defects in l-threonine biosynthesis partially suppress zipA1 mutant thermosensitivity. (A to D) WM5337 and mutants lacking thrA, thrB, or thrC were grown for 1.5 h at 42°C in LB. (E to H) Viability was measured by spotting assay on LB at 30°C (E), 34°C (F), 37°C (G), or 42°C (H) for 24 h. ND, no dilution. (I) The levels of ZipA and FtsZ protein in indicated cells grown at 37°C for 2 h were determined by Western blotting using antibody against ZipA or FtsZ.