TABLE 2.
E. coli strains used in this studya
| Strain | Description | Source |
|---|---|---|
| W3110 | rpoSam rph-1 INV (rrnD-rrnE) | Lab collection |
| WM1074 | Wild-type MG1655 F− λ− ilvG rfb50 rph-1 lacU169 | Lab collection |
| MC4100 | F− [araD139]B/r Δ(argF-lac)169 λ− e14− flhD5301 Δ(fruK-yeiR)725 (fruA25) relA1 rpsL150(Strr) rbsR22 Δ(fimB-fimE)632(::IS1) deoC1 | Lab collection |
| WM2991 | W3110 zipA1 (M44V, T211L, Y229C, and N281S) | |
| WM5322 | WM2991 zipA+ ΔnupC::Tn10 | zipA+, nupC::Tn10 × WM2991 |
| WM5337 | MG1655 zipA1 ΔnupC::Tn10 | zipA1, nupC::Tn10 × MG1655 |
| WM5339 | MG1655 nupC::Tn10 | zipA+, nupC::Tn10 × MG1655 |
| WM5372 | WM5339 ΔthrA::kan | ΔthrA, JW0001-1a × WM5339 |
| WM5376 | WM5337 ΔthrA::kan | ΔthrA, JW0001-1 × WM5337 |
| WM5373 | WM5339 ΔthrB::kan | ΔthrB, JW0002-3 × WM5339 |
| WM5377 | WM5337 ΔthrB::kan | ΔthrB, JW0002-3 × WM5337 |
| WM5351 | WM5339 ΔthrC::kan | ΔthrC, JW0003-2 × WM5339 |
| WM5352 | WM5337 ΔthrC::kan | ΔthrC, JW0003-2 × WM5337 |
| WM5369 | WM5339 ΔglyA::kan | ΔglyA, JW2535-1 × WM5339 |
| WM5354 | WM5337 ΔglyA::kan | ΔglyA, JW2535-1 × WM5337 |
| WM5429 | WM5339 ΔltaE::kan | ΔltaE, JW0854-1 × WM5339 |
| WM5433 | WM5337 ΔltaE::kan | ΔltaE, JW0854-1 × WM5337 |
| WM5374 | WM5339 Δtdh::kan | Δtdh, JW3591-4 × WM5339 |
| WM5378 | WM5337 Δtdh::kan | Δtdh, JW3591-4 × WM5337 |
| WM5375 | WM5339 ΔtdcB::kan | ΔtdcB, JW3088-2 × WM5339 |
| WM5379 | WM5337 ΔtdcB::kan | ΔtdcB, JW3088-2 × WM5337 |
| WM5385 | WM5339 ΔmetA::kan | ΔmetA, JW3973-1 × WM5339 |
| WM5388 | WM5337 ΔmetA::kan | ΔmetA, JW3973-1 × WM5337 |
| WM5386 | WM5339 ΔmetB::kan | ΔmetB, JW3910-1 × WM5339 |
| WM5389 | WM5337 ΔmetB::kan | ΔmetB, JW3910-1 × WM5337 |
| WM5387 | WM5339 ΔmetC::kan | ΔmetC, JW2975-1 × WM5339 |
| WM5390 | WM5337 ΔmetC::kan | ΔmetC, JW2975-1 × WM5337 |
| WM5426 | WM5339 ΔmetE::kan | ΔmetE, JW3805-1 × WM5339 |
| WM5430 | WM5337 ΔmetE::kan | ΔmetE, JW3805-1 × WM5337 |
| WM5474 | WM5339 ΔmetL::kan | ΔmetL, JW3911-1 × WM5339 |
| WM5475 | WM5337 ΔmetL::kan | ΔmetL, JW3911-1 × WM5337 |
| WM5427 | WM5339 ΔmetH::kan | ΔmetH, JW3979-1 × WM5339 |
| WM5431 | WM5337 ΔmetH::kan | ΔmetH, JW3979-1 × WM5337 |
| WM5428 | WM5339 ΔmmuM::kan | ΔmmuM, JW0253-1 × WM5339 |
| WM5432 | WM5337 ΔmmuM::kan | ΔmmuM, JW0253-1 × WM5337 |
| WM5472 | WM5339 ΔilvA::kan | ΔilvA, JW3745-2 × WM5339 |
| WM5473 | WM5337 ΔilvA::kan | ΔilvA, JW3745-2 × WM5337 |
| WM5476 | WM5339 ΔlysC::kan | ΔlysC, JW3984-1 × WM5339 |
| WM5477 | WM5337 ΔlysC::kan | ΔlysC, JW3984-1 × WM5337 |
| WM5693 | WM5337 ΔargD::kan | ΔargD, JW3322-1 × WM5337 |
| WM5694 | WM5337 ΔlysA::kan | ΔlysA, JW2806-1 × WM5337 |
| WM5695 | WM5337 ΔtrpA::kan | ΔtrpA, JW1252-1 × WM5337 |
| WM5696 | WM5337 ΔtrpB::kan | ΔtrpB, JW1253-1 × WM5337 |
| WM5697 | WM5337 ΔmalY::kan | ΔmalY, JW1614-1 × WM5337 |
| WM5698 | WM5337 Δkbl::kan | Δkbl, JW3592-2 × WM5337 |
| WM5705 | WM5337 ΔaspC::kan | ΔaspC, JW0911-1 × WM5337 |
| WM5706 | WM5337 ΔhisC::kan | ΔhisC, JW2003-1 × WM5337 |
| WM5707 | WM5337 ΔhisD::kan | ΔhisD, JW2002-1 × WM5337 |
| WM5708 | WM5337 ΔasnB::kan | ΔasnB, JW0660-2 × WM5337 |
| WM5440 | WM5337 ΔglyA::frt | WM5354 cured by pCP20 |
| WM5441 | WM5337 ΔltaE::frt | WM5433 cured by pCP20 |
| WM5442 | WM5337 Δtdh::frt | WM5378 cured by pCP20 |
| WM5443 | WM5337 ΔtdcB::frt | WM5379 cured by pCP20 |
| WM5444 | WM5337 ΔmetC::frt | WM5390 cured by pCP20 |
| WM5462 | WM5337 ΔltaE::frt ΔtdcB::kan | ΔtdcB, JW3088-2 × WM5441 |
| WM5463 | WM5337 ΔltaE::frt ΔmetC::kan | ΔtdcB, JW3088-2 × WM5444 |
| WM5464 | WM5337 ΔglyA::frt ΔltaE::kan | ΔltaE, JW0854-1 × WM5440 |
| WM5485 | WM1125 (ftsZ84) ΔthrC::kan in WM1074 | ΔthrC, JW0003-2 × WM1125 |
| WM5486 | WM1125 (ftsZ84) ΔmetC::kan in WM1074 | ΔmetC, JW2975-1 × WM1125 |
| WM5487 | WM3993 (ftsI23) ΔthrC::kan in MC4100 | ΔthrC, JW0003-2 × WM3993 |
| WM5488 | WM3993 (ftsI23) ΔmetC::kan in MC4100 | ΔmetC, JW2975-1 × WM3993 |
| WM5489 | WM3994 (ftsQ1) ΔthrC::kan in MC4100 | ΔthrC, JW0003-2 × WM3994 |
| WM5490 | WM3994 (ftsQ1) ΔmetC::kan in MC4100 | ΔmetC, JW2975-1 × WM3994 |
| WM5491 | WM2101 (ftsK44) ΔthrC::kan in WM1074 | ΔthrC, JW0003-2 × WM2101 |
| WM5492 | WM2101 (ftsK44) ΔmetC::kan in WM1074 | ΔmetC, JW2975-1 × WM2101 |
| WM4649 | WM3993 (ftsI23) in WM1074 | ftsI23 leuO::Tn10 × WM1074 |
| WM4661 | WM3994 (ftsQ1) in WM1074 | ftsQ1 leuO::Tn10 × WM1074 |
| WM5817 | WM4649 (ftsI23) ΔthrC::kan in WM1074 | ΔthrC, JW0003-2 × WM4649 |
| WM5818 | WM4649 (ftsI23) ΔmetC::kan in WM1074 | ΔmetC, JW2975-1 × WM4649 |
| WM5819 | WM4661 (ftsQ1) ΔthrC::kan in WM1074 | ΔthrC, JW0003-2 × WM4661 |
| WM5820 | WM4661 (ftsQ1) ΔmetC::kan in WM1074 | ΔmetC, JW2975-1 × WM4661 |
a
Phage P1 lysates were made from the indicated JW strains in the KEIO collection and used as donors in transductions.