TABLE 3.
Phenotypic suppression pattern for lptA42 and opgH103 alleles
| Strain | Chromosomal version of: |
Plasmid |
EOPa |
Row | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| lptA | opgH | lptAb | opgHc | LD | Bct (150 μg/ml) | SDS-EDTA | Rif (2.5 μg/ml) | Nov (10 μg/ml) | ||
| PS103 | ΔlptA | opgH103 | lptA42 | NP | + | + | + | + | ± | 1 |
| PS001 | ΔlptA | opgH+ | lptA+ | NP | + | + | + | + | + | 2 |
| − | + | + | + | + | + | 3 | ||||
| opgH+ | + | + | + | + | + | 4 | ||||
| opgH103 | + | + | + | + | + | 5 | ||||
| PS003 | ΔlptA | opgH+ | lptA41 | NP | + | − | − | − | − | 6 |
| − | + | − | − | − | − | 7 | ||||
| opgH+ | + | − | − | − | − | 8 | ||||
| opgH103 | + | − | − | − | − | 9 | ||||
| PS111 | ΔlptA | opgH+ | lptA42 | NP | + | + | + | − | − | 10 |
| − | + | + | + | − | − | 11 | ||||
| opgH+ | + | + | + | − | − | 12 | ||||
| opgH103 | + | + | + | − | ± | 13 | ||||
| PS112 | ΔlptA | ΔopgH | lptA+ | NP | + | + | + | + | + | 14 |
| − | + | + | + | + | + | 15 | ||||
| opgH+ | + | + | + | + | + | 16 | ||||
| opgH103 | + | + | + | + | + | 17 | ||||
| PS109 | ΔlptA | ΔopgH | lptA41 | NP | + | − | − | − | − | 18 |
| − | + | − | − | − | − | 19 | ||||
| opgH+ | + | − | − | − | − | 20 | ||||
| opgH103 | + | − | − | − | − | 21 | ||||
| PS110 | ΔlptA | ΔopgH | lptA42 | NP | + | + | + | + | ± | 22 |
| − | + | + | + | + | ± | 23 | ||||
| opgH+ | + | + | + | − | − | 24 | ||||
| opgH103 | + | + | + | + | ± | 25 | ||||
a
EOP relative to growth on LD agar. +, EOP > 10−2; ±, EOP between 10−2 and 10−3; −, EOP < 10−3.
b
lptA+, pWSK29-LptA LptB; lptA41, pWSK29-LptA41 LptB; lptA42, pWSK29-LptA42 LptB.
c
NP, no plasmid; −, empty vector pGS100; opgH+, pGS100-OpgH; opgH103, pGS100-OpgH103.