TABLE 6.
E. coli strains
| Strain | Parental strain | Relevant characteristicsa |
Constructionb | Source or reference | |
|---|---|---|---|---|---|
| Chromosomal | Plasmid | ||||
| AM604 | MC4100 | Ara+ | Spontaneous Ara+ revertant | 18 | |
| BL21(DE3) | F− ompT hsdSB(rB− mB−) gal dcm (DE3) | 87 | |||
| BW25113 | laclq rrnBT14 lacZWJ16 hsdR514 araBADAH33 rhaBADLD78 | 76 | |||
| DH10B | araD139Δ(ara leu)7697 ΔlacX74 galU galK rpsL deoR ϕ80dlacZΔM15 endA1 nupG recA1 mcrA(mrr hsdRMS mcrBC) | 88 | |||
| EM001 | JW2343 | ΔmlaA | By FLP-mediated excision of kan cassette | This work | |
| EM004 | BW25113/pACYC184-LptA LptB | ΔlptAB::kan | plac-lptA lptB; Ampr | By site-directed λRed-mediated mutagenesis with ΔlptAB::kan DNA | This work |
| FL907 | AM604 | AM604 ϕ(kan araC araBp-lptA)1 | 16 | ||
| JW2343 | BW25113 | F− Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ− ΔmlaA754::kan rph-1 Δ(rhaD-rhaB)568 hsdR514 | 89 | ||
| PS001 | AM604/pWSK29-LptA LptB | ΔlptAB::kan | plac-lptA lptB; Ampr | By P1 HFT*EM004 transduction, selection for Kanr | This work |
| PS003 | AM604/pWSK29-LptA41 LptB | ΔlptAB::kan | plac-lptA41 lptB; Ampr | By P1 HFT*EM004 transduction, selection for Kanr | This work |
| PS101 | PS003 | ΔlptAB::kan | plac-lptA41 lptB; Ampr | Bctr; spontaneous suppressor mutant | This work |
| PS102 | PS003 | mlaA102 ΔlptAB::kan | plac-lptA41 lptB; Ampr | Bctr, spontaneous suppressor mutant | This work |
| PS103 | PS003 | mdoH103 ΔlptAB::kan | plac-lptA42 lptB; Ampr | Bctr, spontaneous suppressor mutant | This work |
| PS107 | PS134/pWSK29-LptA41LptB | ΔmlaA ΔlptAB::kan | plac-lptA41 lptB; Ampr | By P1 HFT*PS001 transduction, selection for Kanr | This work |
| PS109 | PS132/pWSK29-LptA41 LptB | ΔopgH ΔlptAB::kan | plac-lptA41 lptB; Ampr | By P1 HFT*PS001 transduction, selection for Kanr | This work |
| PS110 | PS132/pWSK29-LptA42 LptB | ΔopgH ΔlptAB::kan | plac-lptA42 lptB; Ampr | By P1 HFT*PS001 transduction, selection for Kanr | This work |
| PS111 | AM604/pWSK29-LptA42 LptB | ΔlptAB::kan | plac-lptA42 lptB; Ampr | By P1 HFT*PS001 transduction, selection for Kanr | This work |
| PS112 | PS132/pWSK29-LptA LptB | ΔopgH ΔlptAB::kan | plac-lptA lptB; Ampr | By P1 HFT*PS001 transduction, selection for Kanr | This work |
| PS113 | PS103 | opgH103 ΔlptAB::kan ΔwcaJ::cat | plac-lptA42 lptB; Ampr | By P1 HFT*PS135 transduction, selection for Catr | This work |
| PS114 | PS109 | ΔopgH ΔlptAB::kan ΔwcaJ::cat | plac-lptA41 lptB; Ampr | By P1 HFT*PS135 transduction, selection for Catr | This work |
| PS115 | PS110 | ΔopgH ΔlptAB::kan ΔwcaJ::cat | plac-lptA42 lptB; Ampr | By P1 HFT*PS135 transduction, selection for Catr | This work |
| PS116 | PS112 | ΔopgH ΔlptAB::kan ΔwcaJ::cat | plac-lptA lptB; Ampr | By P1 HFT*PS135 transduction, selection for Catr | This work |
| PS130 | PS134/pWSK29-LptA LptB | ΔmlaA ΔlptAB::kan | plac-lptA lptB; Ampr | By P1 HFT*PS001 transduction, selection for Kanr | This work |
| PS131 | AM604 | ΔopgH::cat | By site-directed λRed-mediated recombination; primers FG3116/FG3117, template pKD3 | This work | |
| PS132 | PS131 | ΔopgH | By FLP-mediated excision of cat cassette | This work | |
| PS133 | AM604 | ΔmlaA::kan | By P1 HFT*JW2343 transduction (selection for Kanr) | This work | |
| PS134 | PS133 | ΔmlaA | By FLP-mediated excision of kan cassette | This work | |
| PS135 | AM604 | ΔwcaJ::cat | By site-directed λRed-mediated recombination; primers FG3153-FG3154, template pKD3 | This work | |
| XL1-Blue | F− λ− recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZ ΔM15 Tn10(Tetr)] | Agilent Technologies | |||
a
Amp, ampicillin; Tet, tetracycline.
b
Kan, kanamycin; Cat, chloramphenicol acetyltransferase.