TABLE 7.

Plasmids

Plasmid	Parental plasmid/replicon	Relevant characteristicsa	Construction, origin, or reference
pACYC184		pSC101 ori p15A Camr Tetr	90
pACYC184-LptA LptB	pACYC184	plac-lptA lptB Camr	lptAB genes were excised from plasmid pWSK29-LptA LptB and subcloned into BamHI-SalI sites of pACYC184.
pCP20		bla cat; thermosensitive replication	76
pET-LptA41-H	pET21a	pT7-lptA41-His6	lptA41 allele was PCR amplified with AP182 and AP183 and cloned into BamHI-NotI sites of pET-LptA-H.
pET-LptA42-H	pET21a	pT7-lptA42-His6 Ampr	lptA42 was obtained by site-directed mutagenesis with FG3191-FG3192 primers from pET-LptA41-H as the template.
pET-LptA-H	pET21a	pT7-lptA-His6 Ampr	27
pGS100	pGZ119EH	ptac-TIR cat oriVColD	91
pGS100-OpgH	pGS100	ptac-opgH Camr	opgH was PCR amplified with FG3069-FG3070 primers from PS003 genomic DNA and cloned into XbaI-PstI sites of pGS100.
pGS100-OpgH103	pGS100	ptac-opgH103 Camr	opgH103 was PCR amplified with FG3069-FG3070 primers from PS003 genomic DNA and cloned into XbaI-PstI sites of pGS100.
pGS100-MlaA	pGS100	ptac-mlaA Camr	mlaA was PCR amplified with FG3067-FG3068 primers from PS003 genomic DNA and cloned into XbaI-PstI sites of pGS100.
pGS100-MlaA102	pGS100	ptac-mlaA102 Camr	mlaA102 was PCR amplified with FG3067-FG3068 primers from PS102 genomic DNA and cloned into XbaI-PstI sites of pGS100.
pGS103	pGS100	ptac-lptC Camr	91
pGS108	pGS100	ptac-lptC-His6 cat oriVColD	16
pKD3		oriRγ Ampr Camr; source of cat cassette	76
pKD4		oriRγ Ampr Kanr; source of kan cassette	76
pKD46		oriR101 repA101ts araC araBp-λ red bla	76
pWSK29	pBSIISK	pSC101 ori f1 ori lacZa Ampr	92
pWSK29-LptA LptB	pWSK29	plac-lptA41 lptB Ampr	93
pWSK29-LptA36 LptB	pWSK29-LptA LptB	plac-lptAI36A I38A lptB Ampr	By site-directed mutagenesis with primers AP184-AP185
pWSK29-LptA37 LptB	pWSK29-LptA36 LptB	plac-lptA36R76D lptB Ampr	By site-directed mutagenesis with primers AP112-AP113
pWSK29-LptA41 LptB	pWSK29-LptA37 LptB	plac-lptA37K83D lptB Ampr	By site-directed mutagenesis with primers AP186-AP187
pWSK29-LptA42 LptB	pWSK29-LptA41 LptB	plac-lptA41M112I lptB Ampr	Spontaneous mutant

^aCam, chloramphenicol.