FIG 12.

Photomicrographs of F. johnsoniae colonies. Colonies were incubated at 25°C on PY2 agar for 36 h. Photomicrographs were taken with a Photometrics Cool-SNAP_cf² camera mounted on an Olympus IMT-2 phase-contrast microscope. The wild-type strains used were F. johnsoniae UW101 and CJ1827 (rpsl mutant of UW101), and the ΔgldJ mutant was CJ2360. Truncated gldJ chromosomal mutants expressing GldJ proteins of 542 amino acids (GldJ₅₄₂), 548 amino acids (GldJ₅₄₈), and 553 amino acids (GldJ₅₅₃) are also shown. The full-length wild-type GldJ is 561 amino acids in length. In each case, the colony on the left has the empty vector pCP11, and the colony on the right has pMM313, which expresses wild-type GldJ. The scale bar indicates 1 mm and applies to all panels.