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. 2017 Dec 20;200(2):e00362-17. doi: 10.1128/JB.00362-17

FIG 4.

FIG 4

Immunodetection of GldK (A), GldL (B), GldM (C) and GldN (D) in cells of wild-type and mutant F. johnsoniae strains. Cell extracts (15 μg of protein) of wild-type (UW101), gldA mutant (CJ101-288), gldB mutant (CJ569), gldD mutant (CJ282), gldF mutant (CJ787), gldH mutant (CJ1043), gldI mutant (UW102-41), and gldJ mutant (UW102-80) were separated by SDS-PAGE, and GldK, GldL, GldM, and GldN were detected by Western blotting using the appropriate antisera (10, 34). Complemented mutants (indicated by superscript “c”) carried the plasmids indicated in Fig. 3. The ΔgldK mutant (CJ2122), ΔgldL mutant (CJ2157), ΔgldM mutant (CJ2262), and ΔgldNO mutant (CJ2090) were used as controls in panels A, B, C, and D, respectively.