FIG 6.

Immunodetection of GldA, GldJ, and GldK in wild-type cells and in cells of strains with mutations in gldA, gldJ, and gldK. UW101 is the F. johnsoniae type strain. CJ1827 is a streptomycin-resistant rpsl mutant of strain UW101 and is wild type for motility and secretion. The ΔgldJ and ΔgldK mutants are CJ2360 and CJ2122, respectively. “gldA disruption” refers to mutant CJ101-288. “gldA G40R” and “gldA R137C” refer to mutants UW102-9 and UW102-168, respectively, which produce GldA with the indicated amino acid changes. Strains listed with superscript “c” denote complementation with pSA21, which carries wild-type gldA.