FIG 8.

Alignment of C-terminal regions of GldJ proteins. GldJ sequences from 22 members of the phylum Bacteroidetes were aligned using MUSCLE. Dark shading indicates identical amino acids, and light shading indicates similar amino acids. Numbers at the right correspond to the length of each wild-type GldJ protein, including signal peptides. Arrows indicate the C-terminal amino acids of mutant F. johnsoniae GldJ proteins truncated at 542, 547, 548, and 553 aa. Sequences were organized with those most similar to F. johnsoniae GldJ at the top and those least similar at the bottom, with class of organisms within the phylum Bacteroidetes indicated on the left. See Fig. S2 for alignment of full-length GldJ sequences.