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. 2017 Dec 20;17:16. doi: 10.1186/s12895-017-0068-3

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Immunohistochemistry findings. a In situ frequency of cytokines and FoxP3 expression (number of positive cells/mm²). Sections of immunohistochemistry staining for (b) anti-IFN-γ (Santa Cruz, Dallas, TX) (c) anti-IL-4 (Santa Cruz), (d) anti-IL-17 (R&D Systems, Minneapolis, MN) and (e) anti-FoxP3 (Ebioscience, San Diego, CA) monoclonal antibodies of a patient’s lesion biopsy. Slides were labeled with streptavidin–biotin complex (Dako, Carpinteria, CA). b and d, original magnification 200×; a and c, original magnification 400×. Numerous stained (brown) cells are found in (b) and (d), a more modest number is found in (e), while rare stained cells (arrow) were detected in (c)