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. 2017 Dec 20;8:287. doi: 10.1186/s13287-017-0737-5

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Isolation, culture, and characteristics of human orbital adipose-derived stem cells (OASCs) and human corneal endothelial cells (HCECs). a,b OASCs were adherent, spindle-shaped, fibroblast-like cells. c Immunofluorescence staining of vimentin. d Flow cytometric analyses of cell markers in human OASCs (n = 3). Blue lines indicate the OASC group. e Peeled DM layer that contained endothelial cells (left panel) and a magnification micrograph of the DM layer (right panel). f Primary culture of HCECs with OASC-CM (days 4, 9, and 10). g Fibroblastic conversion and expression of CEC relative markers in P6 BM-HCECs. Scale bars = 100 μm