Fig. 4.

Relative cell marker expression in HCECs at different passages cultivated in BM or OASC-CM. RT-PCR analysis of Na⁺/K⁺ ATPase (a), ZO-1 (b), Col8a2 (c), and SLC4A4 (d) (n = 3). e,f Western blotting analysis and protein levels of Na⁺/K⁺ ATPase and ZO-1. Results are means ± SEM (n = 3). *p < 0.05, versus P3 (BM). BM basal medium, CM conditioned medium, Col8a2 collagen type VIII, HCEC human corneal endothelial cell, P passage, SLC4A4 solute carrier family 4 anion exchanger member 4, Zo-1 zonula occludens-1