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. 2017 Dec 20;17:875. doi: 10.1186/s12885-017-3894-0

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Isoprenaline promotes plexin-A1 expression in human gastric cancer cell lines. a-c MGC-803 and HGC-27 cells were treated with 0, 5 or 10 μM ISO for 3 h after serum starvation. a qRT-PCR analysis of plexin-A1 expression in MGC803 and HGC27 cells. b-c The expression of plexin-A1 protein was analyzed by Western blot and quantified. d-f MGC803 cells were starved overnight and treated with 5 μM ISO for 0, 1, 2, 3 and 6 h; the relative mRNA expression levels of plexin-A1 were analyzed using qRT-PCR (d) and the protein expression of plexin-A1 was analyzed by Western blot and quantified (e-f).Data represent mean ± SD (n = 3 for each group,*P < 0.05, **P < 0.01)