Fig. 5.

Molecular identification of reptile-associated Borrelia species in the Goanna tick (Bothriocroton undatum) from Sydney, Australia. a Summary table showing the V1-V3 and V3-V4 16S rRNA gene diversity profiling assay reads for Borrelia OTUs within each tick sample (Tick 1–6) recovered using either Method 1 or Method 2. The table includes the total number of high quality reads, the proportion of Borrelia reads, as well as the library identifier (JS2948-JS2970). b, c Multiple sequence alignment of the overlapping Borrelia OTU region of the V1-V3 (b) and V3-V4 (c) 16S rRNA gene sequence, with Borrelia sp. 16S rRNA gene sequence amplified from Tick2 and Tick3. Identical residues with the top reference are indicated by dots. Both Borrelia spp. Tick2 and Tick3 16S rRNA gene sequences are indistinguishable within the V3-V4 16S rRNA hypervariable region