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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: Mol Biochem Parasitol. 2017 Jun 13;216:5–13. doi: 10.1016/j.molbiopara.2017.06.002

Fig. 5. PF3D7_0402000 (D90c) binds to inside out vesicles (IOVs) in a concentration-dependent manner.

Fig. 5

Western blots of IOV binding assay for A) D90c and B) Mature parasite-infected erythrocyte surface antigen (MESA). E. coli-expressed purified proteins, MBP-D90c-His and MBP-MESA at concentrations ranging from 0.0008 μM to 0.023 μM were separately incubated overnight with 10 μg of erythrocyte IOVs, collected by centrifugation, washed, and subjected to SDS-PAGE analysis and western blotting using anti-MBP antibody. Blots were reprobed with anti-actin antibody to demonstrate equal loading. MESA and MBP-His protein were included as positive and negative controls, respectively. Molecular weight markers (sizes in kDa) are indicated at left.