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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Hum Mutat. 2017 May 2;38(6):658–668. doi: 10.1002/humu.23201

Figure 1. Characterization of the ability of the different SAMHD1 AGS variants to restrict HIV-1 infection. (A).

Figure 1

Figure 1

Figure 1

Human SAMHD1 protein is depicted showing the numbers of the amino acids residues at the boundaries of each domain along with the position and number of each AGS mutant studied here. AGS mutants studies in this work as indicates. The nuclear localization signal, the phosphorylation site on T592, and the Vpx interaction motif are depicted. Human monocytic U937 cells stably expressing the indicated mutant and wild type SAMHD1 proteins (B) were challenged with increasing amounts of HIV-1-GFP. Forthy hours post infection the percentage of GFP-positive cells was determined by flow cytometry (C). As control, U937 cells stably transduced with the empty vector LPCX were challenged with HIV-1-GFP. All SAMHD1 AGS mutant infection experiments were repeated at least 3 times and a representative figure is shown.