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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Hum Mutat. 2017 May 2;38(6):658–668. doi: 10.1002/humu.23201

Figure 3. SAMHD1 AGS mutants binding to nucleic acids.

Figure 3

Human 293T cells were transfected with plasmid expressing the indicated wild type and mutant SAMHD1 protein. Cells were lysed 24 hours after transfection (Input), were incubated with ISD-PS immobilized in StrepTactin Superflow affinity resin. Similarly, eluted proteins from the resin were visualized by Western blotting using anti-FLAG antibodies (Bound). Similar results were obtained in three independent experiments and a representative experiment is shown. ISD-PS, interferon-stimulatory DNA sequence containing a phosphorothioate backbone; WB, Western blot; WT, wild type.