Figure 4.

Key carbon, sulfur and hydrogen metabolisms putatively inferred from the subsurface ANME-2d genome. Arrows show the directions of reaction pathways with compounds and enzymes involved in the reaction pathways as well as ion and electron transports. A gene not found in the other ANME genomes (ANME-1, ANME-2a and nitrate-reducing ANME-2d genomes) but in the subsurface ANME-2d genome is highlighted in red, whereas genes not found in subsurface ANME-2d genome but in nitrate-reducing ANME-2d genomes are shown in gray. Blue arrows show the electron transport hypothesized based on the homology of the Ni-Fe hydrogenases Group 1 gene sequence and that of the sulfite reductase gene sequence. Full names of genes are listed in Supplementary Table S3.