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Genetic characteristics of the Ni-Fe hydrogenases Group 1 in the subsurface ANME-2d genome. (a) Gene organization surrounding a large subunit with Ni-Fe biding motifs. (b) Neighbor-joining tree of Ni-Fe hydrogenase genes based on deduced amino-acid sequences. Bootstrap values with >50% and >75% are indicated by open and solid circles, respectively. Blue and green taxa are known as sulfate and Fe(III) reducers, respectively.
