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N-Acetylation activities of the AAC(6′)-Ib variants and the related resistance against (a) ciprofloxacin, (b) kanamycin and (c) gemifloxacin. The N-acetylation activities of the purified variant enzymes are indicated with white bars. Statistical results (t-test) were provided in Supplementary Table 6. The altered resistance of E. coli DH5α harboring the variant genes is shown based on minimal inhibitory concentration values (black bars) and images of the disk-diffusion assay results.
