| Volume (Liters) | 0.5 | 1 | 1.5 | 2 | 2.5 | 3 | |
|---|---|---|---|---|---|---|---|
| NaCl | 1.5 | 3 | 4.5 | 6 | 7.5 | 9 | g |
| Peptone | 1 | 2 | 3 | 4 | 5 | 6 | g |
| Agar | 8.5 | 17 | 25.5 | 34 | 42.5 | 51 | g |
| 0.5% Cholesterol | 0.5 | 1 | 1.5 | 2 | 2.5 | 3 | ml |
| To be added after autoclaving and cooling, before pouring plates: | |||||||
| 1 M MgSO4 | 0.5 | 1 | 1.5 | 2 | 2.5 | 3 | ml |
| 1 M CaCl2 | 0.5 | 1 | 1.5 | 2 | 2.5 | 3 | ml |
| 1 M KPI | 12.5 | 25 | 37.5 | 50 | 62.5 | 75 | ml |
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