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. 2016 May 9;16(2):244–251. doi: 10.1177/1534735416645182

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© The Author(s) 2016

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page(https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 3. — Downregulation of CXCR4 by OBW occurs at the transcriptional level.

(A) HCT116 cells (1 × 10⁶) were treated with OBW at the indicated concentrations. Total RNA was isolated and analyzed by RT-PCR assays. GAPDH was used to show equal loading of total RNA. *P< .05 versus control. Data are expressed as mean ± SD. (B) HCT116 cells (1 × 10⁶) were treated with OBW at the indicated concentrations. Total RNA was isolated and analyzed by real-time PCR assays. The crossing point (Cp) value of CXCR4 was normalized by keeping the Cp value of GAPDH as a control; bars show the standard error (*P< .05). (C) OBW suppresses constitutive activation of NF-κB in HCT116 cells.Cells were incubated with the indicated concentrations of OBW for 24 hours, and then the nuclear extracts were assayed for NF-κB activation in HCT116cells. *P< .05 versus control. Data are expressed as mean ± SD.