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. 2016 May 5;16(4):496–504. doi: 10.1177/1534735416645184

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© The Author(s) 2016

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page(https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 1. — Chrysin increased DTX-induced cytotoxicity in A549 cells.

(A) Flowchart of drug treatment for cytotoxicity. (B) Combination effect of chrysin on DTX-induced cytotoxicity. Combination index below 1 showed synergic effect.

A549 cells were treated with (A) control, (B) DTX, (C) chrysin, (D) combination of chrysin and DTX. The lysates were applied on apoptosis-related protein antibody-printed membrane according to Materials and Methods. (E) Each spot represented apoptosis-related proteins (n = 2). Gray block showed synergic effect in the combination of chrysin and DTX. Underline represented inhibition of expression in the combination of chrysin and DTX comparing with control, chrysin, or DTX. Abbreviations: Pos, positive control; Neg, negative control; BL, blank.