Figure 2.

β-Lapachone induces apoptosis through extrinsic and intrinsic signaling pathways in CT26 cells. (A) CT26 cells were incubated with the indicated concentrations of β-lapachone for 9 hours and stained with annexin V and PI. The figure is representative of 3 independent experiments. (B) CT26 cells were treated with β-lapachone (1 µM) for 0 to 9 hours. (C) CT26 cells were treated with various concentrations of β-lapachone for 9 hours and subjected to western blotting with antibodies against PARP, caspase-3, -8, -9, Bcl-2, Bcl-xL, and Bax.