Figure 2.

In vitro cytotoxicity assays. (A) MTT assay. Cells were incubated with Icariside II, Icar-MC (1-40 μM) or empty micelles (0.2 mg/mL) for 12, 24, and 48 hours to evaluate cytotoxicity by MTT assay. (B) Lactate dehydrogenase (LDH) release. The cells were treated with Icariside II and Icar-MC for 12 hours, before analyzing the media for LDH release (*P < .05, **P < .01 versus control). (C) Apoptosis. Cells were incubated with Icariside II and Icar-MC (25 µM) for 24 hours to evaluate apoptosis by flow cytometry.