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. 2017 Dec 15;28(26):3741–3755. doi: 10.1091/mbc.E17-01-0026

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© 2017 Gabasa et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Assembly of α-SMA into stress fibers in EMT-competent lung epithelial cells and primary lung fibroblasts on stimulation with TGF-β1 for 3 d. (A) Quantitative colocalization analysis of the images in Figure 3 through the Manders colocalization coefficient M(α-SMA), which corresponds to the fraction (between 0 and 1) of α-SMA co-occurring with F-actin. (B) Colocalization analysis of the images in Figure 3 in terms of the percentage of cells stimulated with TGF-β1 that exhibited at least three straight and continuous white lines (green bars) or three straight and discontinuous (but not speckled) white lines (red bars), which were interpreted as apparent (well-differentiated) myofibroblasts or proto-myofibroblasts, respectively. (C) Absolute average F-actin fluorescence intensity per cell obtained on stimulation with TGF-β1 in epithelial cells undergoing weak or strong EMT and in primary fibroblasts from either control or IPF patients. Statistical analysis as in Figure 2.