FIGURE 6:

ANI-2 regulates the localization of NMY-2, CYK-7, and ANI-1 at the stable PGC cytoplasmic bridge. (A, C, E) Confocal images (maximum intensity projections of five to seven consecutive Z planes) of PGCs in embryos at the 150- to 250-cell-stage expressing either NMY-2::GFP (A), CYK-7::GFP (C), or GFP::ANI-1 (E) and fixed and stained with antibodies against GFP (green), ANI-2 (magenta), germ granules (white), and DAPI (blue). Embryos were depleted of the genes indicated by RNAi. Scale bar, 10 μm. (B, D, F) Quantification of the focus volume of NMY-2::GFP (B), CYK-7::GFP (D), or GFP::ANI-1 (F) at the PGC cytoplasmic bridge in embryos depleted of the indicated genes by RNAi. The red bars represent average ± SD. *: p < 0.05; **: p < 0.01; ***: p < 0.001; n.s.: p > 0.05.