Table 1.

Overview of major next-generation sequencing platforms.

Company	Platform	Amplification	Sequencing	Read length	Throughput/ time per run	Dominant error type	Overall error rate
Roche/454 Life Sciences	GS FLX Titanium XL+	Emulsion PCR	Pyrosequencing	Up to 1 kb	700 Mb/23 h	Indel	0.5%
	GS FLX Titanium XLR70			Up to 600 bp	450 Mb/10 h
	GS Junior			~400 bp	35 Mb/10 h
	HiSeq 2000			36–100 bp	105–600 Gb/2– 11 days
Illumina	Genome Analyzer IIx	Bridge PCR	Sequencing-by- synthesis with reversible terminator	35–150 bp	10–95 Gb/2– 14 days	Substitution	0.2%
	MiSeq			36–250 bp	540 Mb–8.5 Gb/4– 39 h
Life Technologies/ Applied Biosystems	5500xl SOLiD™ system	Emulsion PCR	Sequencing by ligation	35–75 bp	10–15 Gb/day	Substitution	0.1%
	SOLiD™ 4 system			25–50 bp	25–100 Gb/3.5– 16 days
Life Technologies/Ion Torrent	Ion Proton™ sequencer (Proton I chip)	Emulsion PCR	Ion semiconductor sequencing	Up to 200 bp	Up to 10 Gb/2–4 h	Indel	1%
	Ion PGM™ sequencer (318 chip)			35–200 bp	300 Mb–1 Gb/0.9– 4.5 h
Helicos BioSciences	HeliScope™ single molecule sequencer	NONE	Single molecule sequencing	25–55 bp	21–35 Gb/8 days	Deletion	5%
Pacific Biosciences	PacBio RS	NONE	Single molecule sequencing	250 bp– 10 kb	NA	Indel	15%