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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Int J Mass Spectrom. 2017 Aug 24;422:42–50. doi: 10.1016/j.ijms.2017.08.004

Table 2. The effect of D-labeling on the evolution of the C2D/C2H ratio.

After D-water labeling, different domains within the crypt demonstrate different degrees of D-labeling, providing an opportunity to assess the evolution of the C2D/C2H ratio as a function of sputtering depth. An analysis of unlabeled crypt (Figures 2,3) is provided as a reference (right columns). The mean ratio is provided for the first plateau (< 3.5*1016 Cs) and the second plateau.



D-labeled
D-unlabeled
C2D/C2H ± s.d.m.
C2D/C2H ± s.d.m.
plateau 1 plateau 2 % change plateau 1 plateau 2 % change


Resin 1.76±0.16 2.46±0.13 40 1.74±0.28 2.55±0.21 46
Cytoplasm Low 4.35±0.48 5.28±0.26 20 2.02±0.22 3.98±0.21 96
High 6.53±0.56 7.52±0.28 15
Granule 19.6±2.9 19.4±1.43 1 2.24±0.28 4.83±0.24 115