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SDS-PAGE of ISY proteins. A. All four ISY proteins from Nepeta after expression optimization and purification by Ni-NTA chromatography and gel filtration chromatography. Despite extensive optimization of purification conditions (see Experimental), proteins were not entirely homogenous. B. SDS-PAGE of N. mussinii ISY 2 after Ni-NTA and gel filtration chromatography used for kinetic analysis.
