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. 2018 Jan;145:48–56. doi: 10.1016/j.phytochem.2017.10.004

Table 2.

Primers used for cloning. Overhangs used for pOPINF InFusion are in bold.

Gene Direction Sequence 5′-3′
NmISY1 Forward AAGTTCTGTTTCAGGGCCCGAGCTGGTGGTGGG
NmISY1 Reverse ATGGTCTAGAAAGCTTTAGGGAACAATCTTGAAAGCTTTCAC
NmISY2 Forward AAGTTCTGTTTCAGGGCCCGAGCATGAGCTTGAGCTGG
NmISY2 Reverse ATGGTCTAGAAAGCTTTAGGTATAGGTAGAAGGAACAAGGTTGTAAG
NcISY1 Forward AAGTTCTGTTTCAGGGCCCGAGCTGGTGGTGGGC
NcISY1 Reverse ATGGTCTAGAAAGCTTTAGGGAACAATCTTGAAAGCTTTCAC
NcISY2 Forward AAGTTCTGTTTCAGGGCCCGAGCATGAACTGGTGGAGG
NcISY2 Reverse ATGGTCTAGAAAGCTTTAAGAAATAGTAGAGGAAGGAACAATCTTGTAAGC